PASEF enhanced DIA#

Summary#

Name	Pub date	Instrument	Lab & Co.	Window overlap
diaPASEF [1]	2020 June	timsTOF Pro	Mann Lab	None
Synchro-PASEF [2]	2022 Dec.	timsTOF Pro 2	Mann Lab	Intra-/inter-frame
IM-GPF [3]	2023 Mar.	timsTOF Pro	Collins Lab	None
Slice-PASEF [4]	2022	timsTOF Pro 2	Demichev Lab	Inter-cycle
midiaPASEF [5]	2022	timsTOF Pro 2	Tenzer Lab	Intra-/inter-frame

Note

Pub date
- the date the method was publicly released by publisher
- Slice-PASEF is a pre-print
- midiaPASEF is a pre-print in 2023 and has a granted patent in 2022
Instrument
- initially developped on which instrument
Lab & Co.
- lab or company (the last corresponding author)

Individuals#

diaPASEF#

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Reference:

Full name	diaPASEF
Authors	Florian Meier et al.
Lab & Company	Mann Lab
Publication date	2020, Oct.
MS instrument	timsTOF Pro

Scheme 1:

Name	16 diaPASEF scans (standard scheme)
Frames per cycle	16 (2 rows)
MS2 spectra per frame	4
Covered m/z range	400-1200 m/z
MS2 window m/z size	25 m/z
Covered IM range (1/K0)	0.6-1.4 Vs/cm²
MS2 window IM size (1/K0)	Variable
Injection times	1
Cycle time	/
MS2 window features	Consecutive & fixed window size & non-overlapping windows

Scheme 2:

Name	8 diaPASEF scans (high speed scheme)
Frames per cycle	8
MS2 spectra per frame	3
Covered m/z range	400-1200 m/z
MS2 window m/z size	25 m/z
Covered IM range (1/K0)	0.6-1.4 Vs/cm²
MS2 window IM size (1/K0)	Variable
Injection times	1
Cycle time	~0.9 s
MS2 window features	Consecutive & fixed window size & non-overlapping windows

Scheme 3:

Name	4 diaPASEF scans (high sensitivity scheme)
Frames per cycle	4
MS2 spectra per frame	4
Covered m/z range	400-1200 m/z
MS2 window m/z size	50 m/z
Covered IM range (1/K0)	0.6-1.4 Vs/cm²
MS2 window IM size (1/K0)	Variable
Injection times	1
Cycle time	/
MS2 window features	Consecutive & fixed window size & non-overlapping windows

Scheme 4:

Name	Single diaPASEF scan
Frames per cycle	1
MS2 spectra per frame	12
Covered m/z range	300-1200 m/z
MS2 window m/z size	Variable
Covered IM range (1/K0)	0.6-1.4 Vs/cm²
MS2 window IM size (1/K0)	Variable
Injection times	1
Cycle time	/
MS2 window features	Consecutive & variable window sizes & non-overlapping windows

Synchro-PASEF#

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Reference:

Full name	Synchro-PASEF
Authors	Patricia Skowronek et al.
Lab & Company	Mann Lab
Publication date	2022 Dec.
MS instrument	timsTOF Pro 2

Scheme 1:

Name	1
Frames per cycle	4
MS2 spectra per frame	927 (100 ms TIMS ramp time)
Covered m/z range	335-1270 m/z
MS2 window m/z size	25 m/z
Quadrupole shift m/z	~0.9 m/z ((1170 - 335) / 927)
Covered IM range (1/K0)	0.7-1.3 Vs/cm²
MS2 window IM size (1/K0)	~7e-4 Vs/cm²
Injection times	1
Cycle time	~0.56 s
MS2 window features	Consecutive & fixed window size & intra-/inter-frame overlapping windows

Scheme 2:

Name	2
Frames per cycle	7
MS2 spectra per frame	927 (100 ms TIMS ramp time)
Covered m/z range	335-1275 m/z
MS2 window m/z size	15 m/z
Covered IM range (1/K0)	0.7-1.3 Vs/cm²
MS2 window IM size (1/K0)	~7e-4 Vs/cm²
Injection times	1
Cycle time	~0.9 s
MS2 window features	Consecutive & fixed window size & intra-/inter-frame overlapping windows

Scheme 3:

Name	3
Frames per cycle	4
MS2 spectra per frame	927 (100 ms TIMS ramp time)
Covered m/z range	335-1305 m/z
MS2 window m/z size	35 m/z, 25 m/z, 25 m/z, 50 m/z
Covered IM range (1/K0)	0.7-1.3 Vs/cm²
MS2 window IM size (1/K0)	~7e-4 Vs/cm²
Injection times	1
Cycle time	~0.56 s
MS2 window features	Consecutive & fixed window size (in one frame) & intra-/inter-frame overlapping windows

IM-GPF#

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Reference:

Full name	IM-GPF
Authors	Jack Penny et al.
Lab & Company	Collins Lab
Publication date	2023, Mar.
MS instrument	timsTOF Pro

Scheme 1:

Name
Frames per cycle	14
MS2 spectra per frame	2
Covered m/z range	400-1200 m/z
MS2 window m/z size	5 m/z
Covered IM range (1/K0)	0.57-1.47 Vs/cm²
MS2 window IM size (1/K0)	/
Injection times	7
Cycle time	/
MS2 window features	Discrete & fixed window size & margin-overlapping windows (1 m/z)

Slice-PASEF#

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Reference:

Full name	Slice-PASEF
Authors	Lukasz Szyrwiel et al.
Lab & Company	Demichev Lab
Publication date	2022
MS instrument	timsTOF Pro 2

Scheme 1:

Name	1-frame (1F)
Frames per cycle	1
MS2 spectra per frame	15
Covered m/z range	400-1000 m/z
MS2 window m/z size	83.74-215.05 m/z
Demultiplexing region m/z	/
Covered IM range (1/K0)	0.75-1.2 Vs/cm²
MS2 window IM size (1/K0)	0.03 Vs/cm²
Injection times	1
Cycle time	/
MS2 window features	Consecutive & variable window size & inter-cycle overlapping windows

Scheme 2:

Name	2-frame (2F)
Frames per cycle	2
MS2 spectra per frame	10
Covered m/z range	400-1000 m/z
MS2 window m/z size	sub-cycle 1: 20.56-162 m/z; sub-cycle 2: 36.55-136.48 m/z; sub-cycle 3: 31.55-128.05 m/z
Demultiplexing region m/z	variable
Covered IM range (1/K0)	0.75-1.2 Vs/cm²
MS2 window IM size (1/K0)	0.045 Vs/cm²
Injection times	1
Cycle time	/
MS2 window features	Consecutive & variable window size & inter-cycle overlapping windows

Scheme 3:

Name	4-frame (4F)
Frames per cycle	4
MS2 spectra per frame	10
Covered m/z range	400-1000 m/z
MS2 window m/z size	sub-cycle 1: 2.75-115.65 m/z; sub-cycle 2: 12.37-105.96 m/z; sub-cycle 3: 19.1-94.2 m/z; sub-cycle 4: 4.82-84.55 m/z
Demultiplexing region m/z	variable
Covered IM range (1/K0)	0.75-1.2 Vs/cm²
MS2 window IM size (1/K0)	0.045 Vs/cm²
Injection times	1
Cycle time	/
MS2 window features	Consecutive & variable window size & inter-cycle overlapping windows

midiaPASEF#

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Reference:

Full name	maxmizing information content in diaPASEF
Authors	Ute Distler et al.
Lab & Company	Tenzer Lab
Publication date	2022 Dec.
MS instrument	timsTOF Pro 2

Scheme 1:

Name
Frames per cycle	20
MS2 spectra per frame	927 (100 ms TIMS ramp time)
Covered m/z range	121-1535 m/z
MS2 window m/z size	36 m/z
Demultiplexing region m/z	12 m/z
Quadrupole shift m/z	~1.24 m/z ((1535 - 20 * 12 - 24 - 121) / 927)
Covered IM range (1/K0)	0.6-1.56 Vs/cm²
MS2 window IM size (1/K0)	~7e-4 Vs/cm²
Injection times	1
Cycle time	~2.2 s
MS2 window features	Consecutive & fixed window size & intra-/inter-frame overlapping windows